Buckner will not play in Sunday's pivotal AFC South matchup against the Tennessee Titans as a result.
Indianapolis will now likely be down two starting defensive linemen in Week 12, with Denico Autry also being placed on the reserve list as a close contact.
Buckner has racked up 41 tackles, 2.5 sacks, one forced fumble, and a fumble recovery through 10 games in an impactful first year with the Colts.
Both teams currently sit tied atop the division with a 7-3 record. Frank Reich's squad earned a 34-17 win over the Titans in their last meeting, which came in Week 10.