The team revealed its lineup before Wednesday's series finale against Milwaukee, and Trout isn't starting.
The 27-year-old didn't need an MRI and is listed as day-to-day. The Angels have an off day on Thursday, and the seven-time All-Star could return to the lineup as early as Friday against the Chicago Cubs.
The center fielder appeared to tweak his groin after sliding feet first into second base on a force out in the second inning on Tuesday. Trout stayed in the game to play defense during the next inning, but Peter Bourjos replaced him in the field to start the fourth.
"He said there might have been a little soreness from a workout that he'd had either earlier today or yesterday, but we can't lose that guy for any length of time, so it was really my call," Halos manager Brad Ausmus said after the 11-8 victory, according to Rhett Bollinger of MLB.com. "He lobbied to stay in and I said we can't take the chance."
Brian Goodwin will replace Trout in center field on Wednesday, when the Angels will try to keep a five-game winning streak alive.
"I wasn't worried about it," Trout told Bollinger. "Then I got in the dugout, I still felt it a little bit. I went in the outfield, started getting a little worse. Just didn't want to push it."
Trout has landed on the injured list twice in his career, though never with a groin issue. He was held to 114 games in 2017 due to a torn ligament in his thumb, and missed a handful of games in 2018 because of a wrist injury.
Through 12 games this season, Trout is 13-for-32 at the plate with five home runs and one stolen base.